FlexiTube siRNAs siH19-2, and AllStars Negative Control siRNA (QIAGEN, Hilden, Germany), 20 µg each, were transfected with lipofectamine 2000 (Thermo Fisher Scientific, Dreieich, Germany) according to the instructions of the manufacturer.
Growth protocol
Cells were cultured in DMEM/high glucose (Sigma, Deisenhoffen, Germany), which was supplemented with 10% FCS (Sigma) and 25mmol/L HEPES (Thermo Fisher, Dreieich, Germany)
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated with the RNeasy Mini Kit (QIAGEN, Hilden, Germany).
Label
biotin
Label protocol
Affymetrix protocol
Hybridization protocol
Microarray analyses was performed at the Microarray-Analytic Center of the Medical Faculty Mannheim, using Clariom™ D Assays (Thermo Fisher Scientific, Dreieich, Germany).
Scan protocol
Microarray analyses was performed at the Microarray-Analytic Center of the Medical Faculty Mannheim, using Clariom™ D Assays (Thermo Fisher Scientific, Dreieich, Germany).
Description
Gene expression data
Data processing
Microarray analyses was performed at the Microarray-Analytic Center of the Medical Faculty Mannheim, using Clariom™ D Assays (Thermo Fisher Scientific, Dreieich, Germany).
