|
| Status |
Public on Oct 31, 2023 |
| Title |
Liver_obese_T2D T2D16 |
| Sample type |
RNA |
| |
|
| Source name |
liver biopsy of an obese human subject with type-2-dabetes
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: liver
condition: type-2-diabetic
age: 51
bmi: 52.01
gender: m
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from snap-frozen liver using the miRNeasy mini kit (Qiagen, Germany) according to the manufacturer’s instructions blinded.
|
| Label |
biotin
|
| Label protocol |
Biotinylated RNA was prepared according to the FlashTag Biotin HSR RNA Labeling Kit (Applied Biosystems, USA) from 500 ng total RNA.
|
| |
|
| Hybridization protocol |
After preparation of the hybridization cocktail using additionaly 3 nM control oligo B2 and hybridiztion controls (GeneChip Hybridization Control Kit, Applied Biosystems), 21.5 µl biotin-labeld sample were injected into one GeneChip miRNA 4.0 Array, multi-species (Applied Biosystems) per sample. Hybridization was performed for 18 h at 48 °C and 60 rpm. Arrays were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChip miRNA 4.0 Array, multi-species were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Liver_obese_T2D
noncoding RNA expression from diabetic and non-diabetic liver of human obese subjects
|
| Data processing |
Data analysis was performed with the Transcriptome Analysis Console software (version 4.0.2.15) with the analysis type 'Expression (Gene)'. As summarization method 'RMA+DABG (Human Only)' was used for signal normalization. ANOVA method 'ebayes' was used for comparison. A probe set was considered expressed if at least 50% of the detection above background (DABG) values were below the DABG threshold.
|
| |
|
| Submission date |
Jun 02, 2021 |
| Last update date |
Oct 31, 2023 |
| Contact name |
Henriette Kirchner |
| E-mail(s) |
henriette.kirchner@uni-luebeck.de
|
| Organization name |
University of Lübeck
|
| Department |
Institute for Human Genetics
|
| Lab |
Epigenetics and Metabolism
|
| Street address |
Ratzeburger Allee 160
|
| City |
Lübeck |
| ZIP/Postal code |
23562 |
| Country |
Germany |
| |
|
| Platform ID |
GPL21572 |
| Series (1) |
| GSE176025 |
Liver microRNA transcriptome reveals miR-182 as link between type 2 diabetes and fatty liver disease in obesity |
|
