|
| Status |
Public on Jun 04, 2021 |
| Title |
35_PB_DLBCL |
| Sample type |
RNA |
| |
|
| Source name |
PB_DLBCL
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
tissue: FFPE
status after 5 years: Alive
age: 70
ann arbor stage: IE
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from fresh frozen with TRIzol (Invitrogen) and Rneasy Mini Kit (Qiagen) and from deparaffinized 10 μm sections of FFPE tissues with the Tissue Preparation System (TPS) robot (Siemens Healthcare Diagnostics). RNA was quantifies with the Qubit RNA BR Assay kit with the Qubit Fluorometer (Invitrogen).
|
| Label |
Fluorescent tag barcodes
|
| Label protocol |
See manufacturer's protocol for the NanoString nCounter Assay System
|
| |
|
| Hybridization protocol |
See manufacturer's protocol for the NanoString nCounter Assay System
|
| Scan protocol |
See manufacturer's protocol for the NanoString nCounter Assay System
|
| Description |
Osseous_32
|
| Data processing |
Data was log2 transformed and standardized by geometric mean, followed by normalization with the housekeeping genes using the 'NanoStringNorm' package in R. Further analysis was additionally performed with R.
|
| |
|
| Submission date |
Jun 03, 2021 |
| Last update date |
Jun 04, 2021 |
| Contact name |
Ruben de Groen |
| E-mail(s) |
r.a.l.de_groen@lumc.nl
|
| Organization name |
Leiden University Medical Center
|
| Department |
Hematology
|
| Street address |
Albinusdreef 2
|
| City |
Leiden |
| ZIP/Postal code |
2333ZA |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL30227 |
| Series (1) |
| GSE176126 |
Gene-expression profiling of osseous and non-osseous DLBCL with the NanoString nCounter technology |
|
