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Status |
Public on Jun 08, 2021 |
Title |
adipose 1month control, rep2 |
Sample type |
RNA |
|
|
Source name |
rat adipose tissue
|
Organism |
Rattus norvegicus |
Characteristics |
strain: Wistar tissue: Adipose tissue gender: Male dietary restriction: None time: 1 month
|
Growth protocol |
5-week-old rats were purchased from Japan SLC (Tokyo, Japan).
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was prepared using RNeasy Plant Mini Kit (Qiagen) according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
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|
|
Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Gene Array Scanner.
|
Description |
a-1m-C(2) Rats fed control diet.
|
Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
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|
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Submission date |
Jun 07, 2021 |
Last update date |
Jun 08, 2021 |
Contact name |
Kenji Saito |
E-mail(s) |
kkkj774@yahoo.co.jp
|
Phone |
+81-3-5841-5114
|
Organization name |
The Univeristy of Tokyo
|
Department |
Department of Applied Biological Chemistry
|
Lab |
Nutritional Biochemistry
|
Street address |
1-1-1, yayoi
|
City |
bunkyo-ku |
ZIP/Postal code |
113-8657 |
Country |
Japan |
|
|
Platform ID |
GPL341 |
Series (2) |
GSE176295 |
Mild food restriction and gene expression profile (adipose) |
GSE176300 |
Mild food restriction and gene expression profile |
|