High-quality DNA from PBMCs was extracted prepared using standard protocols (Bisulfite conversion Qiagen, hybridization, and Infinium Methylation Assay Illumina)
Label
cy3,cy5
Label protocol
Illumina protcol
Hybridization protocol
Illumina protcol
Scan protocol
Illumina protcol
Data processing
R package minfi was used to transform the raw data normalized and generate M value hg19