NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM537142 Query DataSets for GSM537142
Status Public on Oct 23, 2010
Title 1-muscle_SCI_Day5
Sample type RNA
 
Source name vastus lateralis, SCI
Organism Homo sapiens
Characteristics human sci: Day 5
tissue: vastus lateralis
Treatment protocol Nine male patients and one female patient with spinal cord injury (SCI) (Mean ± SEM = 43.9 ± 6.7 yrs) were recruited for this study.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted according to the standard Affymetrix protocol including Trizol extraction and Rneasy column cleanup
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 plus2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 800.
 
Submission date Apr 23, 2010
Last update date Apr 23, 2010
Contact name Yi-Wen Chen
E-mail(s) ychen@cnmc.org
Organization name Children's Research Institute
Department Genetic Medicine Research
Street address 111 Michigan Ave NW
City Washington
State/province DC
ZIP/Postal code 20010
Country USA
 
Platform ID GPL570
Series (1)
GSE21497 Common Gene Transcriptional Patterns Following Immobilization, Spinal Cord Injury, and Unloading in Human Skeletal Muscle

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 2610.95 P 0.000296708
AFFX-BioB-M_at 6231.77 P 4.42873e-05
AFFX-BioB-3_at 3037.62 P 7.00668e-05
AFFX-BioC-5_at 6997.2 P 4.42873e-05
AFFX-BioC-3_at 9078.56 P 7.00668e-05
AFFX-BioDn-5_at 8520.18 P 4.42873e-05
AFFX-BioDn-3_at 43934.2 P 4.42873e-05
AFFX-CreX-5_at 99509.9 P 5.16732e-05
AFFX-CreX-3_at 136913 P 4.42873e-05
AFFX-DapX-5_at 6.55049 A 0.945787
AFFX-DapX-M_at 40.5807 A 0.645547
AFFX-DapX-3_at 129.93 A 0.860518
AFFX-LysX-5_at 25.0876 A 0.904333
AFFX-LysX-M_at 35.9553 A 0.86866
AFFX-LysX-3_at 70.2446 A 0.672921
AFFX-PheX-5_at 131.87 A 0.300606
AFFX-PheX-M_at 18.312 A 0.966323
AFFX-PheX-3_at 50.8313 A 0.574038
AFFX-ThrX-5_at 120.302 A 0.41138
AFFX-ThrX-M_at 11.5513 A 0.976071

Total number of rows: 54675

Table truncated, full table size 1616 Kbytes.




Supplementary file Size Download File type/resource
GSM537142.CEL.gz 4.1 Mb (ftp)(http) CEL
GSM537142.CHP.gz 496.6 Kb (ftp)(http) CHP
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap