|
| Status |
Public on Oct 23, 2010 |
| Title |
10-muscle_SCI_Day5 |
| Sample type |
RNA |
| |
|
| Source name |
vastus lateralis, SCI
|
| Organism |
Homo sapiens |
| Characteristics |
human sci: Day 5
tissue: vastus lateralis
|
| Treatment protocol |
Nine male patients and one female patient with spinal cord injury (SCI) (Mean ± SEM = 43.9 ± 6.7 yrs) were recruited for this study.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted according to the standard Affymetrix protocol including Trizol extraction and Rneasy column cleanup
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 plus2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 800.
|
| |
|
| Submission date |
Apr 23, 2010 |
| Last update date |
Apr 23, 2010 |
| Contact name |
Yi-Wen Chen |
| E-mail(s) |
ychen@cnmc.org
|
| Organization name |
Children's Research Institute
|
| Department |
Genetic Medicine Research
|
| Street address |
111 Michigan Ave NW
|
| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20010 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE21497 |
Common Gene Transcriptional Patterns Following Immobilization, Spinal Cord Injury, and Unloading in Human Skeletal Muscle |
|
