|
| Status |
Public on Jul 07, 2010 |
| Title |
16 week_Lpr_CD4+T cells |
| Sample type |
RNA |
| |
|
| Source name |
16 week MRL/lpr primary CD4+T cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: MRL/lpr
disease state: lupus
tissue: spleen
cell type: CD4+T cells
age: 16 weeks
|
| Treatment protocol |
n/a
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mirVana miRNA Isolation Kit, see http://www.ambion.com/catalog/CatNum.php?1560
|
| Label |
FAM
|
| Label protocol |
n/a
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
the purity of cell >94%
pool of 6 MRL/lpr mouse CD4+T cells
|
| Data processing |
TLDA were run in the 7900 HT Sequence Detection system. The ABI TaqMan SDS v2.3 software was utilized to obtain raw CT values. Automatic baseline and manual CT were set to 0.2 for all samples. Input data for class comparison, permutations and prediction analysis consisted of the raw miR expression CT values normalized to the endogenous housekeeping snoRNA202. [delta] CT = CT_sample - CT_reference.
|
| |
|
| Submission date |
Apr 23, 2010 |
| Last update date |
Jul 07, 2010 |
| Contact name |
Nan Shen |
| E-mail(s) |
nanshensibs@gmail.com
|
| Organization name |
Renji Hospital, JiaoTong University School of Medicine
|
| Department |
Department of Rheumatology
|
| Street address |
145 Shan Dong Middle Road
|
| City |
Shanghai |
| ZIP/Postal code |
200001 |
| Country |
China |
| |
|
| Platform ID |
GPL10354 |
| Series (1) |
| GSE21220 |
miRNAs expression in the splenic CD4+ T cells and B cells isolated from MRL/lpr mice at 5 and 16 weeks of age |
|
