|
| Status |
Public on Jan 04, 2022 |
| Title |
model brain Replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
brain,model 7days
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: brain
strain: male
|
| Growth protocol |
Drink and eat freely
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
10 µg of total RNA were primed with 2 µl of 100 µM T16N2 DNA primer at 70°C for 10 min, then reversed transcribed at 42°C for 1 h in the presence of 400 U SuperScript II RTase (Invitrogen), and 100 µM each dATP, dTTP, dGTP, with 25 µM dCTP, 25 µM Cy3-label
|
| |
|
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| Scan protocol |
Scanned on an Agilent G2505C scanner.
Images were quantified using Agilent Feature Extraction Software (version A.10.7.1.1).
|
| Description |
Biological replicate 1 of 4. middle cerebral artery embolization model rat hippocampus.7days.
|
| Data processing |
Agilent Feature Extraction Software (v 10.7.1.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Jun 17, 2021 |
| Last update date |
Jan 04, 2022 |
| Contact name |
Bo-wei CHEN |
| E-mail(s) |
201207020217@stu.hnucm.edu.cn
|
| Phone |
86-0731-85366925
|
| Organization name |
Hunan University of Chinese Medicine
|
| Street address |
No. 95 Shaoshan Middle Road
|
| City |
Changsha |
| ZIP/Postal code |
410007 |
| Country |
China |
| |
|
| Platform ID |
GPL30293 |
| Series (1) |
| GSE178439 |
Effects of Buyang Huanwu Decoction on circRNA-miRNA-mRNA transcriptional network of rats with cerebral ischemia |
|
