|
| Status |
Public on Jun 01, 2022 |
| Title |
U251P_Carm_1 |
| Sample type |
SRA |
| |
|
| Source name |
U251P
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: U251P
treatment: Carmofur
replicate #: 1
|
| Treatment protocol |
For these samples, cells were seeded and allowed to adhere. Then, they were serum starved overnight (16hr) followed by 24hr in the presence of the full media with DMSO or carmofur. Cell pellets were isolated at the end of that 24hr.
|
| Growth protocol |
Gibco phenol red-free DMEM/F12 containing 2.5 mM L-glutamine and 17.5 mM glucose and supplemented with 10% FBS, B27 equivalent-Gem 21 (Gemini Biosciences), 20 ng/ml epidermal growth factor and fibroblast growth factor basic, 1% sodium pyruvate, and 1% penicillin/streptomycin. 5% CO2 and 21% O2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Total RNA Extration Kit with DNAse (Norgen)
QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Lexogen's QuantSeq Bluebee data analysis pipeline used for data processing
Genome_build: hg38
Supplementary_files_format_and_content: raw count table at the gene level for all 12 samples
|
| |
|
| Submission date |
Jun 28, 2021 |
| Last update date |
Jun 01, 2022 |
| Contact name |
Sara Cooper |
| E-mail(s) |
sjcooper@hudsonalpha.org
|
| Organization name |
HudsonAlpha Institute for Biotechnology
|
| Street address |
601 Genome Way
|
| City |
Huntsville |
| State/province |
AL |
| ZIP/Postal code |
35806 |
| Country |
USA |
| |
|
| Platform ID |
GPL18573 |
| Series (1) |
| GSE179087 |
Targeting the Sphingolipid Balance via Acid Ceramidase Inhibition toDecrease Growth of TMZ-Resistant Glioblastoma and Block Migration |
|
| Relations |
| BioSample |
SAMN19926724 |
| SRA |
SRX11246572 |
