|
| Status |
Public on Nov 11, 2021 |
| Title |
mPFC LSD rep1 |
| Sample type |
RNA |
| |
|
| Source name |
mPFC LSD
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
Sex: male
tissue: medial prefrontal cortex
treatment: LSD
|
| Treatment protocol |
(+)-LSD tartrate was obtained from NIDA and dissolved at a concentration of 1.0 mg/ml in sterile water. Four rats were administered 1.0 mg/kg LSD i.p., and four rats were simultaneously administered i.p. saline. After 90 min the animals were decapitated. The brains were removed and the prefrontal cortex, hippocampus and midbrain were quickly dissected, frozen in liquid nitrogen, and stored at –80°C until processing.
|
| Growth protocol |
Male Sprague-Dawley rats (250-275g) were purchased from Harlan and maintained for at least one week prior to use. Rats were given ad libitum access to food and water and maintained on a 12 h light/dark cycle.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the tissue of four animals pooled per treatment group. Pooling of the tissues should help average out differences in individual responses to the drug. RNA isolated from the prefrontal cortex of LSD and saline treated rats was purified using the RNeasy kit from Qiagen (Valencia, CA).
|
| Label |
biotin
|
| Label protocol |
Used BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics; Farmingdale, NY) as per the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Biotin labeled cRNA was provided to Research Genetics (Huntsville, AL) for hybridization and analysis with the Affymetrix rat U34A array set.
|
| Scan protocol |
Arrays were scanned.
|
| Description |
LSD_rep1
Rat's mPFC after 90min of i.p. Saline administration
|
| Data processing |
The GeneChip ® Analysis software package from Affymetrix was employed by Research Genetics to identify statistically significant changes in gene expression.
|
| |
|
| Submission date |
Jul 03, 2021 |
| Last update date |
Nov 11, 2021 |
| Contact name |
Aurora Savino |
| E-mail(s) |
aurora.savino@unito.it
|
| Organization name |
Università di Torino
|
| Street address |
via Nizza, 52
|
| City |
Torino |
| State/province |
Piemonte |
| ZIP/Postal code |
10126 |
| Country |
Italy |
| |
|
| Platform ID |
GPL85 |
| Series (2) |
| GSE179378 |
A single dose of lysergic acid diethylamide influences gene expression patterns within the mammalian brain [array] |
| GSE179380 |
LSD influences gene expression patterns within the mammalian brain |
|
