|
| Status |
Public on Sep 01, 2021 |
| Title |
D28_preg_1 |
| Sample type |
SRA |
| |
|
| Source name |
pregnant mare
|
| Organism |
Equus caballus |
| Characteristics |
tissue: endometrium
Sex: female
days after ovulation: 28
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RNEasy Mini kit. TruSeq Stranded mRNA Library Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Sequenced reads were trimmed for adaptor sequence and low quality ends and reads shorter than 50 bp were removed
Reads were then mapped to the equine EquCab3 genome allowing two mismatches per read
Total counts and transcripts per million (TPM) were generated for all transcripts
Genome_build: EquCab3
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample
|
| |
|
| Submission date |
Jul 13, 2021 |
| Last update date |
Sep 01, 2021 |
| Contact name |
Claudia Klein |
| E-mail(s) |
claudia.klein@fli.de
|
| Phone |
050348715135
|
| Organization name |
Friedrich-Loeffler-Institut, Institute of Farm Animal Genetics
|
| Street address |
Höltystr. 10
|
| City |
Neustadt |
| ZIP/Postal code |
31535 |
| Country |
Germany |
| |
|
| Platform ID |
GPL21401 |
| Series (1) |
| GSE180022 |
Transcriptional profiling of equine endometrium before, during and after capsule disintegration during normal pregnancy and after oxytocin-induced luteostasis in non-pregnant mares |
|
| Relations |
| BioSample |
SAMN20199235 |
| SRA |
SRX11431737 |
