|
| Status |
Public on Mar 31, 2011 |
| Title |
MCF-7 cells-control, biological rep 4 |
| Sample type |
RNA |
| |
|
| Source name |
control MCF-7 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF-7
agent: control
|
| Treatment protocol |
Cells were treated in complete medium with DMSO (control) or 40 uM gamma tocotrienol for 24h
|
| Growth protocol |
MCF-7 cells were grown in 100 mm dishes in DMEM with 5% FBS at 37oC with 5%CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using trizol following manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix U133A2 GeneChip Array following manufacturer's instructions.
|
| Scan protocol |
GeneChip Scanner 3000
|
| Description |
Gene expression data from MCF-7 cells treated with vehicle control (DMSO)
|
| Data processing |
RMA for background adjustment, quantile method for normalization, and a robust multi-array average for summarization
|
| |
|
| Submission date |
May 21, 2010 |
| Last update date |
Mar 31, 2011 |
| Contact name |
Habtom Ressom |
| Organization name |
Georgetown University
|
| Street address |
4000 Reservoir Rd.
|
| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
DC |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE21946 |
Expression data of MCF-7 cells treated with gamma tocotrienol (g-T3) |
|
