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Status |
Public on Mar 31, 2011 |
Title |
MCF-7 cells-40mM g-T3, biological rep 1 |
Sample type |
RNA |
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|
Source name |
treated MCF-7 cells
|
Organism |
Homo sapiens |
Characteristics |
cell line: MCF-7 agent: gamma tocotrienol
|
Treatment protocol |
Cells were treated in complete medium with DMSO (control) or 40 uM gamma tocotrienol for 24h
|
Growth protocol |
MCF-7 cells were grown in 100 mm dishes in DMEM with 5% FBS at 37oC with 5%CO2
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated using trizol following manufacturer's instructions
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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|
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Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix U133A2 GeneChip Array following manufacturer's instructions.
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Scan protocol |
GeneChip Scanner 3000
|
Description |
Gene expression data from MCF-7 cells treated with gamma tocotrienol
|
Data processing |
RMA for background adjustment, quantile method for normalization, and a robust multi-array average for summarization
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Submission date |
May 21, 2010 |
Last update date |
Mar 31, 2011 |
Contact name |
Habtom Ressom |
Organization name |
Georgetown University
|
Street address |
4000 Reservoir Rd.
|
City |
Washington |
State/province |
DC |
ZIP/Postal code |
DC |
Country |
USA |
|
|
Platform ID |
GPL571 |
Series (1) |
GSE21946 |
Expression data of MCF-7 cells treated with gamma tocotrienol (g-T3) |
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