|
Status |
Public on May 22, 2024 |
Title |
006T |
Sample type |
SRA |
|
|
Source name |
Renal papillary cell carcinoma
|
Organism |
Homo sapiens |
Characteristics |
disease state: Tumor tissue: Normal renal tissue
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was harvested using Trizol reagent. 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
RNA-Seq paired-end clean data obtained were then analyzed using Hisat2, Samtools-1.9, Stringtie 1.3.5 and DESeq2. Human reference genome (Homo_sapiens.GRCh38.84.gtf.gz) was obtained from Ensembl (ftp://ftp.ensembl.org/pub/release-84/gtf/homo_sapiens/Homo_sapiens.GRCh38.84.gtf.gz). The genes annotated by GENCODE version 22 were used. Genome_build: Homo_sapiens.GRCh38.84.gtf Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample
|
|
|
Submission date |
Jul 25, 2021 |
Last update date |
May 22, 2024 |
Contact name |
jinhuan wei |
E-mail(s) |
weijh23@mail.sysu.edu.cn
|
Organization name |
SYSU
|
Street address |
No.58 zhongshan 2rd Road
|
City |
Guangzhou |
ZIP/Postal code |
510080 |
Country |
China |
|
|
Platform ID |
GPL24676 |
Series (1) |
|
Relations |
BioSample |
SAMN20378264 |
SRA |
SRX11547938 |