|
| Status |
Public on Jul 01, 2022 |
| Title |
Ectopic expression of SRRM4 in HEK293 cells |
| Sample type |
SRA |
| |
|
| Source name |
HEK 293 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HEK293
genotype: Ectopic expression of SRRM4
|
| Treatment protocol |
1 ug/mL of Doxycycline induction for 24 h.
|
| Growth protocol |
Cell lines were cultured at 37°C and 5% CO2 in DMEM (H3BE12-604F/U1, Lonza Group AG [Cultek S.L.U, Madrid, Spain]) supplemented with 10% tetracycline-free FBS (631106, Clontech, [Conda Laboratories, Torrejon de Ardoz, Spain]) and 1% penicillin/streptomycin (15140122, Life Technologies S.A., [Fisher Scientific, Madrid, Spain]).
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNeasy Mini kit (QIAGEN).
RNA libraries were prepared for sequencing using standard Illumina protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
CL_293_L_SRRM4_OE
PolyA-selected stranded RNA-seq.
|
| Data processing |
Raw reads were mapped with vast-tools align v2.5.1.
Genome_build: hg38 (GRCh38)
Supplementary_files_format_and_content: *.cRPKM.txt: Tab-delimited text files with raw read counts and cRPKMs.
|
| |
|
| Submission date |
Jul 25, 2021 |
| Last update date |
Jul 01, 2022 |
| Contact name |
Manuel Irimia |
| E-mail(s) |
mirimia@gmail.com
|
| Phone |
+34933160212
|
| Organization name |
Centre for Genomic Regulation
|
| Department |
Systems Biology Unit
|
| Street address |
88 Dr. Aiguader
|
| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
|
| Platform ID |
GPL16791 |
| Series (2) |
| GSE180779 |
Regulation of retina microexons by SRRM3, SRRM4 and MSI1 in human cell lines |
| GSE180781 |
Regulation of retina microexons by srrm3 in zebrafish and by SRRM3, SRRM4 and MSI1 in human cell lines |
|
| Relations |
| BioSample |
SAMN20378415 |
| SRA |
SRX11547907 |
