|
| Status |
Public on Jul 26, 2021 |
| Title |
No polyIC WT_2__IFE |
| Sample type |
SRA |
| |
|
| Source name |
Hair follicles
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: skin
experimental group: saline
genotyping: wild type
|
| Treatment protocol |
Epidermis from C57BL/6 mice were harvested one day after saline or poly(I:C) i.p. injection and interfollicular epidermis (IFE) and bulge cells were sorted into Trizol.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Direct-zol RNA MicroPrep (Zymo Research).
RNA libraries were prepared using a NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs), NEBNext Ultra RNA Library Prep Kit for Illumina (New England BioLabs) and NEBNext Multiplex Oligos for Illumina (Index Primers Set) (New England BioLabs) according to manufacturer protocol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Data processing |
The FASTQ files were aligned to the reference mouse genome (mm10) using STAR software in Partek Flow and gene-level count matrix was generated.
Genome_build: mm10
|
| |
|
| Submission date |
Jul 25, 2021 |
| Last update date |
Jul 26, 2021 |
| Contact name |
Keisuke Chris Nagao |
| E-mail(s) |
keisuke.nagao@nih.gov
|
| Organization name |
National Institute of Arthritis and Musculoskeletal and Skin Diseases
|
| Lab |
Cutaneous Leukocyte Biology Section
|
| Street address |
9000 Rockville Pike, Bldg 10, Rm12N240B
|
| City |
Bethesda |
| State/province |
Maryland |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL21493 |
| Series (1) |
| GSE180803 |
Transcriptomic profiles of type I/III interferon-responsive keratinocyte subsets from WT with or without Poly:IC injection |
|
| Relations |
| BioSample |
SAMN20391663 |
| SRA |
SRX11548687 |
