|
| Status |
Public on Mar 01, 2023 |
| Title |
BSCHF_163 |
| Sample type |
RNA |
| |
|
| Source name |
whole blood
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: whole blood
diagnosis: Chronic Heart Failure (CHF)
Sex: Male
age: 79
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PAXgene tubes (Qiagen) and RNA was isolated using TRizol® (Life Technologies) and dissolved in 20 μl RNAse-free water.
|
| Label |
biotin
|
| Label protocol |
The RNA was processed to single-stranded sense fragmented DNA using the GeneChip® WT PLUS Reagent Kit which relies on a reverse transcription priming strategy that primes both poly-A and non-poly-A RNA
|
| |
|
| Hybridization protocol |
5 μg of fragmented, end–labelled sense strand target cDNA was hybridized to each array and scanned using a Gene Chip Scanner 30007G
|
| Scan protocol |
standard protocol
|
| Description |
human whole blood RNA profile
|
| Data processing |
The normalisation used the method by Welsh et al called IRON. BMC Bioinformatics. 2013 May 7;14:153. doi: 10.1186/1471-2105-14-153.
|
| |
|
| Submission date |
Jul 29, 2021 |
| Last update date |
Mar 01, 2023 |
| Contact name |
James A Timmons |
| E-mail(s) |
jamie.timmons@gmail.com
|
| Organization name |
Augur Precision Medicine LTD
|
| Street address |
Cambridge
|
| City |
Cambridge |
| ZIP/Postal code |
CB1 |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL30456 |
| Series (1) |
| GSE181114 |
Whole blood transcriptomic profiling in men and women with heart failure |
|
