|
Status |
Public on Mar 01, 2023 |
Title |
BSCHF_482 |
Sample type |
RNA |
|
|
Source name |
whole blood
|
Organism |
Homo sapiens |
Characteristics |
tissue: whole blood diagnosis: Chronic Heart Failure (CHF) Sex: Female age: 71
|
Extracted molecule |
total RNA |
Extraction protocol |
PAXgene tubes (Qiagen) and RNA was isolated using TRizol® (Life Technologies) and dissolved in 20 μl RNAse-free water.
|
Label |
biotin
|
Label protocol |
The RNA was processed to single-stranded sense fragmented DNA using the GeneChip® WT PLUS Reagent Kit which relies on a reverse transcription priming strategy that primes both poly-A and non-poly-A RNA
|
|
|
Hybridization protocol |
5 μg of fragmented, end–labelled sense strand target cDNA was hybridized to each array and scanned using a Gene Chip Scanner 30007G
|
Scan protocol |
standard protocol
|
Description |
human whole blood RNA profile
|
Data processing |
The normalisation used the method by Welsh et al called IRON. BMC Bioinformatics. 2013 May 7;14:153. doi: 10.1186/1471-2105-14-153.
|
|
|
Submission date |
Jul 29, 2021 |
Last update date |
Mar 01, 2023 |
Contact name |
James A Timmons |
E-mail(s) |
jamie.timmons@gmail.com
|
Organization name |
Augur Precision Medicine LTD
|
Street address |
Cambridge
|
City |
Cambridge |
ZIP/Postal code |
CB1 |
Country |
United Kingdom |
|
|
Platform ID |
GPL30456 |
Series (1) |
GSE181114 |
Whole blood transcriptomic profiling in men and women with heart failure |
|