|
| Status |
Public on Nov 03, 2022 |
| Title |
1 Vehicle |
| Sample type |
SRA |
| |
|
| Source name |
Human Primary Periphheral Blood B Cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: B cells
activating antibody: Vehicle
treatment: 8 hours incubation
|
| Treatment protocol |
anti-CD74 activating antibody was added to 10^7 cells for 1 hour. Then samples 1 Vehicle, 1 CD74 activated, 2 Vehicle and 2 CD74 activated underwent IP with anti-H3K4me2 antibody (ab-32356 ).
|
| Growth protocol |
Primary B cells were isolated from freshly received blood donations.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
In-house ChIP-Seq protocol, Libraries were evaluated by Qubit and TapeStation. Sequencing libraries were constructed with barcodes to allow multiplexing of y samples on one lane.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
1_H3_Ctrl
|
| Data processing |
Reads were demultiplexed
Adapters were trimmed using the cutadapt tool
The reads were aligned uniquely to the human genome (hg19)
Bound regions were detected using MACS2
Genome_build: hg19
Supplementary_files_format_and_content: bed files of MACS2 peaks
|
| |
|
| Submission date |
Aug 09, 2021 |
| Last update date |
Nov 03, 2022 |
| Contact name |
Idit Shachar |
| Organization name |
Weizmann Institute
|
| Street address |
234 Herzl Street
|
| City |
Rehovot |
| ZIP/Postal code |
POB 26 7610001 |
| Country |
Israel |
| |
|
| Platform ID |
GPL18573 |
| Series (2) |
| GSE181676 |
Genome wide H3K4me2 detection after CD74 activation. |
| GSE181677 |
B cells after CD74 activation. |
|
| Relations |
| BioSample |
SAMN20669181 |
| SRA |
SRX11675273 |
