|
| Status |
Public on Jun 08, 2010 |
| Title |
MM13 |
| Sample type |
RNA |
| |
|
| Source name |
Melanoma metastasis
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Melanoma metastasis
os (days): 14
event (0=alive, 1=dead): 1
sex: Male
age at metastases: 51
type of metastases: Subcutaneous
age at primary diagnosis: 50
localization of primary melanoma: Trunk
type: NMM
breslow: 4.5
clark: 4
stage: IV
braf/nras: NRAS-Q61L
cdkn2a (hd=homozygous deletion, *=germline): HD
molecular subtype: Proliferative
cd3 immunohistochemistry: non-brisk
cd20 immunohistochemistry: absent
ki67 (0=<30%, 1=>30%): 1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using qiazol followed by Qiagen RNAeasy kit
|
| Label |
biotin
|
| Label protocol |
RNA was amplified and labelled according to Illuminas recommendations
|
| |
|
| Hybridization protocol |
Arrays were hybridizedaccording to Illuminas recommendations
|
| Scan protocol |
Arrays were scanned according to Illuminas recommendations
|
| Data processing |
Images were analysed using Beadstudio v3 and normalized using the cubic-spline method. VALUE represents Beadstudio processed, cubic spline normalised intensity data from Refseq genes that were used in subsequent analyses.
|
| |
|
| Submission date |
Jun 04, 2010 |
| Last update date |
Jun 07, 2010 |
| Contact name |
Johan Staaf |
| Organization name |
SCIBLU - Swegene Centre for Integrative Biology at Lund University
|
| Street address |
Medicon Village
|
| City |
Lund |
| ZIP/Postal code |
SE-223 81 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL6102 |
| Series (2) |
| GSE22153 |
Gene Experssion Profiling-Based Identification of Molecular Subtypes in Stage IV Melanoma with Different Clinical Outcome (test set) |
| GSE22155 |
Gene Expression Profiling-Based Identification of Molecular Subtypes in Stage IV Melanoma with Different Clinical Outcome |
|
