|
| Status |
Public on Oct 23, 2021 |
| Title |
biological rep3 for sro 4-5mm inflorescence |
| Sample type |
RNA |
| |
|
| Source name |
inflorescence in 4-5mm length
|
| Organism |
Oryza sativa |
| Characteristics |
genotype: the sro mutant
tissue: rice inflorescence
|
| Treatment protocol |
No special treatment
|
| Growth protocol |
Rice was grown in the paddy field
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
boitin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng total RNA (3_ivt_express_kit_manual Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 7G.
|
| Description |
Microarray data of genome-wide gene expression profile
|
| Data processing |
The data were analyzed with PARTEK genomic suit 6.4 using RMA normalization
|
| |
|
| Submission date |
Aug 09, 2021 |
| Last update date |
Oct 23, 2021 |
| Contact name |
Xu wei |
| E-mail(s) |
xuwei1983128@126.com
|
| Phone |
15900726273
|
| Organization name |
SJTU
|
| Department |
Life Science
|
| Lab |
Zhang Dabing
|
| Street address |
800 Dong Chuan Road
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200240 |
| Country |
China |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE181736 |
Expression data from rice inflorescences of the sro mutant and wild type |
|
