|
Status |
Public on Jun 08, 2010 |
Title |
MM86 |
Sample type |
RNA |
|
|
Source name |
Melanoma metastasis
|
Organism |
Homo sapiens |
Characteristics |
tissue: Melanoma metastasis os (days): 836 event (0=alive, 1=dead): 0 sex: Male age at metastases: 61 type of metastases: Lymphnode age at primary diagnosis: 58 localization of primary melanoma: Trunk type: NMM breslow: 1.8 clark: 4 stage: IV braf/nras: BRAF-V600E cdkn2a (hd=homozygous deletion, *=germline): - molecular subtype: Pigmentation cd3 immunohistochemistry: brisk cd20 immunohistochemistry: brisk ki67 (0=<30%, 1=>30%): 0
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using qiazol followed by Qiagen RNAeasy kit
|
Label |
biotin
|
Label protocol |
RNA was amplified and labelled according to Illuminas recommendations
|
|
|
Hybridization protocol |
Arrays were hybridizedaccording to Illuminas recommendations
|
Scan protocol |
Arrays were scanned according to Illuminas recommendations
|
Data processing |
Images were analysed using Beadstudio v3 and normalized using the cubic-spline method. VALUE represents Beadstudio processed, cubic spline normalised intensity data from Refseq genes that were used in subsequent analyses.
|
|
|
Submission date |
Jun 04, 2010 |
Last update date |
Jun 07, 2010 |
Contact name |
Johan Staaf |
Organization name |
SCIBLU - Swegene Centre for Integrative Biology at Lund University
|
Street address |
Medicon Village
|
City |
Lund |
ZIP/Postal code |
SE-223 81 |
Country |
Sweden |
|
|
Platform ID |
GPL6102 |
Series (2) |
GSE22153 |
Gene Experssion Profiling-Based Identification of Molecular Subtypes in Stage IV Melanoma with Different Clinical Outcome (test set) |
GSE22155 |
Gene Expression Profiling-Based Identification of Molecular Subtypes in Stage IV Melanoma with Different Clinical Outcome |
|