|
| Status |
Public on Aug 28, 2021 |
| Title |
Afp1 rep-3 |
| Sample type |
SRA |
| |
|
| Source name |
Asexual spore and mycelium
|
| Organism |
Aspergillus fumigatus Af293 |
| Characteristics |
strain: AF293.1
genotype: afp1 deletion mutant
tissue: conidia and mycelium
|
| Treatment protocol |
Freeze-dry
|
| Growth protocol |
Incubate 2 days in liquid media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent (Invitrogen). 500 ng of total RNA was used for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using LEXOGEN Quant-Seq Library Prep Kit (Cat#001.24) standard protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Illumina Casava1.8 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence(using bbmap), then mapped to hg19 whole genome using Bowtie2
Read count extraction and nomalization were performed using edgeR.
Genome_build: Aspergillus fumigatus Af293
Supplementary_files_format_and_content: tab-delimited text files include read count values for each Sample
|
| |
|
| Submission date |
Aug 26, 2021 |
| Last update date |
Aug 30, 2021 |
| Contact name |
Kwang-Soo Shin |
| E-mail(s) |
shinks@dju.kr
|
| Phone |
82-42-280-2439
|
| Organization name |
Daejeon University
|
| Department |
Life Science
|
| Street address |
96-3
|
| City |
Daejeon |
| ZIP/Postal code |
300-716 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL30552 |
| Series (1) |
| GSE182799 |
Histone acetyl transferase (HAT) and APSES TF of Aspergillus fumigatus |
|
| Relations |
| BioSample |
SAMN20983308 |
| SRA |
SRX11913675 |
