|
| Status |
Public on Jun 17, 2010 |
| Title |
small RNA_chip_A_thaliana_leaf2 |
| Sample type |
RNA |
| |
|
| Source name |
Young Leaf
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
sample type: Arabidopsis thaliana 4x
tissue: Young Leaf
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Small RNAs were enriched from total RNA using mirRVana isolation kit (Ambion, Austin, TX).
|
| Label |
Cy5
|
| Label protocol |
Small RNAs labeled with Cy5 (Mirus Bio, Madison, WI) overnight at 37°C.
|
| |
|
| Hybridization protocol |
After prehybridization, the chip was hybridized to small RNAs labeled with Cy5 (Mirus Bio, Madison, WI) overnight at 37°C. Posthybridization washes were done sequentially once each with 6XSSPET and 3XSSPET and twice with 0.5XSSPET.
|
| Scan protocol |
Hybridized chip was scanned using a Genepix 4000B (Molecular Devices, Sunnyvale, CA), and data were extracted using software provided by CombiMatrix (Mukilteo, WA).
|
| Description |
small RNA
A total of 12 hybridization intensities in three biological replicates per a species were obtained, and log values of hybridization intensities were used for normalization and statistical analysis.
|
| Data processing |
If the hybridization intensities of a miRNA were not significantly higher than the background signals, the miRNA was considered to be not expressed.
|
| |
|
| Submission date |
Jun 10, 2010 |
| Last update date |
Jun 16, 2010 |
| Contact name |
Misook Ha |
| E-mail(s) |
misook.ha@gmail.com
|
| Phone |
7732795900
|
| Organization name |
National Heart Lung Blood Institute
|
| Lab |
Laboratory of Epigenome Biology
|
| Street address |
NIH
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL8814 |
| Series (1) |
| GSE15443 |
Small RNAs serve as a genetic buffer against genomic shock in Arabidopsis interspecific hybrids and allopolyploids |
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