|
| Status |
Public on Aug 29, 2012 |
| Title |
Total RNA from closed bud before flower blooming |
| Sample type |
RNA |
| |
|
| Source name |
Closed bud before flower opens
|
| Organism |
Solanum lycopersicum |
| Characteristics |
tissue: closed bud before flower opens (including developing sepals, stamens petals and carpels)
genotype: Solanum lycopersicum cv. Micro-Tom
developmental stage: Closed bud before flower opens
|
| Treatment protocol |
none
|
| Growth protocol |
Plants were grown in pots with soil in a growth chamber having a photoperiod of 16/8h day/night, a thermoperiod 23/ 18ºC day/night and humidity of 40%.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from Solanum lycopersicum cv. Micro-Tom using a phenol chloroform procedure.
|
| Label |
biotin
|
| Label protocol |
GeneChip 3' IVT Express kit. the user manual is P/N 702646 Rev.1
|
| |
|
| Hybridization protocol |
The samples were hybridised in the standard way, 16hrs at 45C and stainned with the Affymetix hyb wash and stain kit.
|
| Scan protocol |
Standard Affymetrix scanning protocol.
|
| Description |
Total RNAs from Solanum lycopersicum cv. Micro-Tom from closed bud before flower opens
|
| Data processing |
The data were analyzed with Bioconductor affy package, using for background correction - RMA, normalization - quantiles, summarization - median polish. The default probe groupping was used.
|
| |
|
| Submission date |
Jun 11, 2010 |
| Last update date |
Aug 30, 2012 |
| Contact name |
Irina Mohorianu |
| E-mail(s) |
irina.mohorianu@paediatrics.ox.ac.uk
|
| Organization name |
University of Oxford
|
| Department |
Medical Sciences Division
|
| Lab |
Oxford Vaccine Group
|
| Street address |
Headington
|
| City |
Oxford |
| ZIP/Postal code |
OX3 7LE |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL4741 |
| Series (1) |
| GSE22300 |
Microarray analysis of gene expression from different developmental stages of the tomato fruit |
|
