|
| Status |
Public on Sep 30, 2021 |
| Title |
Gudjonsson_021_173 |
| Sample type |
RNA |
| |
|
| Source name |
Skin
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Skin
disease state: Pityriasis_Rubra_Pilaris
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from 10μm sections of formalin-fixed paraffin embedded blocks of identified skin biopsies. RNA was extracted using the E.N.Z.A. FFPE RNA Kit (Omega Bio-tek).
|
| Label |
biotin
|
| Label protocol |
Complementary DNA was prepared and biotinylated using the NuGEN Encore Biotin Module (Encore Biotin Module Manual, P/N M01111 v6).
|
| |
|
| Hybridization protocol |
Labeled cDNA was hybridized at 48°C to Affymetrix Human Gene ST 2.1 array plates, which were then washed, stained and scanned using the Affymetrix GeneTitan system (software version 3.2.4.1515) with the assistance of the University of Michigan DNA Sequencing Core.
|
| Scan protocol |
Labeled cDNA was hybridized at 48°C to Affymetrix Human Gene ST 2.1 array plates, which were then washed, stained and scanned using the Affymetrix GeneTitan system (software version 3.2.4.1515) with the assistance of the University of Michigan DNA Sequencing Core.
|
| Data processing |
R bioconduct was used to process the data, and RMA was used to summarize and normalize the expression values
|
| |
|
| Submission date |
Aug 31, 2021 |
| Last update date |
Oct 01, 2021 |
| Contact name |
Lam Tsoi |
| E-mail(s) |
alextsoi@umich.edu
|
| Organization name |
University of Michigan
|
| Street address |
Medical Sciences 1 Bldg. 1301 E. Catherine
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL30572 |
| Series (1) |
| GSE183134 |
Transcriptomic profiling of Pityriasis Rubra Pilaris (PRP) and Psoriasis |
|
