|
| Status |
Public on Sep 15, 2021 |
| Title |
TGF-β- T98G - T1 |
| Sample type |
RNA |
| |
|
| Source name |
T98G GBM cells
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Brain
|
| Treatment protocol |
T98G cells were treated with 10 ng/ml of TGF-β1 for 24 h
|
| Growth protocol |
T98G glioblastoma cells were cultured in in complete medium, DMEM (Invitrogen) containing 10% fetal bovine serum (FBS) supplemented with 1 mm l-glutamine and penicillin/streptomycin (Gibco).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using MN Nucleospin RNA plus isolation kit (Cat.No:740984.5). 10 µg of purified RNA samples were treated with recombinant DNAse1 (Invitrogen Thermo Scientific - Cat.NO: EN0521) as per manufacturer’s instructions, and the RNA samples were column purified using MN Nucleospin column purification kit.
|
| Label |
Cy3
|
| Label protocol |
Total RNA was end-labeled using Agilent Quick-Amp labeling Kit (p/n5190-0442)
|
| |
|
| Hybridization protocol |
End labelled RNA was hybridized to Agilent Human Gene Expression Microarray 8X60K. Fragmentation of labeled cRNA and hybridization were done using the Gene Expression Hybridization kit of (Agilent Technologies, In situ Hybridization kit, # 5190-0404).
|
| Scan protocol |
The hybridized slides were scanned using the Agilent Microarray Scanner (Agilent Technologies, Part Number G2600D)
|
| Description |
Gene expression after 24h of TGF-β treatment
|
| Data processing |
Data extraction from Images was done using Feature Extraction software and Normalization of the data was done in GeneSpring GX : 75th percentile shift method and gene based analysis was performed
Log base 2
|
| |
|
| Submission date |
Sep 01, 2021 |
| Last update date |
Sep 16, 2021 |
| Contact name |
Genotypic technology |
| E-mail(s) |
sudha.rao@genotypic.co.in
|
| Organization name |
Genotypic Technology
|
| Street address |
259, Apoorva 4th cross,80 feet Road,RMV 2ND STAGE
|
| City |
Bangalore |
| State/province |
Karnataka |
| ZIP/Postal code |
560094 |
| Country |
India |
| |
|
| Platform ID |
GPL20844 |
| Series (1) |
| GSE183211 |
Identification of TGF-β regulated lncRNAs in GBM cells using microarray screen |
|
