|
| Status |
Public on Jan 25, 2023 |
| Title |
D8_CK138: D8 TTNtv/+ AMG2591713 treated CMW |
| Sample type |
SRA |
| |
|
| Source name |
cardiac microwire culture
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: cardiac microwire culture
treatment: AMG2591713
genotype: TTNtv/+
|
| Treatment protocol |
14 days
|
| Growth protocol |
300 μl of CMW maintenance mediumcontaining DMEM/F12, 2% fetal bovine serum, 10 μg/ml insulin and 1xantibiotic/antimycotic (all from ThermoFisher)was gently added to each well for continuous culturing. Medium is changed every 2-3 days by very slowly removing halfof medium from the side of the well at half-way heightand through the drop-wise addition of halfof fresh CMW maintenance medium.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
cardiac microwires were dissociated into a single cell suspension and encapsulated with the 10x Chromium
Samples were processed using the 10x Genomics 3'v3 kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
reads were aligned and count tables generated using cellranger v 4.0.0
Genome_build: GRCh38 (hg38)
Supplementary_files_format_and_content: barcode tsv files include cellbarcodes, features tsv files include gene identifiers, matrix files are the cell by gene count table encoded as a sparse matrix in the Market Exchange Format (MEX)
|
| |
|
| Submission date |
Sep 01, 2021 |
| Last update date |
Jan 25, 2023 |
| Contact name |
Tracy Yamawaki |
| E-mail(s) |
tyamawak@amgen.com
|
| Organization name |
AMGEN, INC.
|
| Street address |
1120 VETERANS BLVD.
|
| City |
SOUTH SAN FRANCISCO |
| State/province |
California |
| ZIP/Postal code |
94080 |
| Country |
USA |
| |
|
| Platform ID |
GPL24676 |
| Series (2) |
| GSE183217 |
Titin truncating variantsin hiPSC-cardiomyocytes inducepathogenic proteinopathy, sarcomeredefect and contractile dysfunction independent of the core contractilemachinery [scRNA-seq] |
| GSE183218 |
Titin truncating variantsin hiPSC-cardiomyocytes inducepathogenic proteinopathy, sarcomeredefect and contractile dysfunction independent of the core contractilemachinery |
|
| Relations |
| BioSample |
SAMN21173669 |
| SRA |
SRX11987484 |
