|
Status |
Public on Jan 15, 2022 |
Title |
RMY7_7 DPI_young_BALF |
Sample type |
SRA |
|
|
Source name |
Bronchoalveolar lavage
|
Organism |
Macaca mulatta |
Characteristics |
tissue: Bronchoalveolar lavage age: young animal_id: RMY7 days post infection: 7 DPI
|
Treatment protocol |
SARS-CoV-2 inoculated Rhesus macaques. Each animal received 2.6e6 TCID15 of total virus through a combined inoculation route of intranasal, intratracheal, oral, and oculated routes.
|
Extracted molecule |
polyA RNA |
Extraction protocol |
10X Genomics version 3.1 reagents were used to construct libraries
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
NextSeq 550 |
|
|
Description |
10X Genomics single-cell RNA Sequencing
|
Data processing |
reads demultiplexed and bcl to fastq conversion using cellranger mkfastq v3.0.2 reads aligned and counted using cellranger count v3.0.2 Genome_build: GCA_003339765.3 & NC_045512.2 Supplementary_files_format_and_content: features.tsv, barcodes.tsv, and matrix.mtx (set of 3 files)
|
|
|
Submission date |
Sep 07, 2021 |
Last update date |
Jan 15, 2022 |
Contact name |
Craig Martens |
E-mail(s) |
martensc@niaid.nih.gov
|
Phone |
406-363-9415
|
Organization name |
NIAID
|
Department |
DIR
|
Lab |
RTB
|
Street address |
903 S. 4th St
|
City |
Hamilton |
State/province |
MT |
ZIP/Postal code |
59840 |
Country |
USA |
|
|
Platform ID |
GPL29319 |
Series (1) |
GSE183579 |
Age-related differences in immune dynamics during SARS-CoV-2 infection in rhesus macaques |
|
Relations |
BioSample |
SAMN21302762 |
SRA |
SRX12031069 |