|
| Status |
Public on Jan 15, 2022 |
| Title |
RMY4_3 DPI_young_BALF |
| Sample type |
SRA |
| |
|
| Source name |
Bronchoalveolar lavage
|
| Organism |
Macaca mulatta |
| Characteristics |
tissue: Bronchoalveolar lavage
age: young
animal_id: RMY4
days post infection: 3 DPI
|
| Treatment protocol |
SARS-CoV-2 inoculated Rhesus macaques. Each animal received 2.6e6 TCID15 of total virus through a combined inoculation route of intranasal, intratracheal, oral, and oculated routes.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
10X Genomics version 3.1 reagents were used to construct libraries
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 550 |
| |
|
| Description |
10X Genomics single-cell RNA Sequencing
|
| Data processing |
reads demultiplexed and bcl to fastq conversion using cellranger mkfastq v3.0.2
reads aligned and counted using cellranger count v3.0.2
Genome_build: GCA_003339765.3 & NC_045512.2
Supplementary_files_format_and_content: features.tsv, barcodes.tsv, and matrix.mtx (set of 3 files)
|
| |
|
| Submission date |
Sep 07, 2021 |
| Last update date |
Jan 15, 2022 |
| Contact name |
Craig Martens |
| E-mail(s) |
martensc@niaid.nih.gov
|
| Phone |
406-363-9415
|
| Organization name |
NIAID
|
| Department |
DIR
|
| Lab |
RTB
|
| Street address |
903 S. 4th St
|
| City |
Hamilton |
| State/province |
MT |
| ZIP/Postal code |
59840 |
| Country |
USA |
| |
|
| Platform ID |
GPL29319 |
| Series (1) |
| GSE183579 |
Age-related differences in immune dynamics during SARS-CoV-2 infection in rhesus macaques |
|
| Relations |
| BioSample |
SAMN21302791 |
| SRA |
SRX12031087 |
