|
| Status |
Public on Oct 01, 2010 |
| Title |
A549 treated with formaldehyde 3 |
| Sample type |
RNA |
| |
|
| Source name |
A549 cells
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: 1 ppm formaldehyde
cell line: A549
|
| Treatment protocol |
Cells at air-liquid interface exposed to 1 ppm formaldehyde for 4 hrs
|
| Growth protocol |
A549 cells were grown in F-12K
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen miRNeasy kit
|
| Label |
Cy3
|
| Label protocol |
Labeled cRNA were prepared according to the standard Agilent protocol
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized on Agilent Human miRNA Microarray, ver 1
|
| Scan protocol |
Microarrays were scanned using Agilent Microarray Scanner
|
| Description |
miRNA expression data from lung cells treated with formaldehyde
|
| Data processing |
data were lowess normalized using Agilent software
|
| |
|
| Submission date |
Jun 15, 2010 |
| Last update date |
Nov 13, 2013 |
| Contact name |
Rebecca Catherine Fry |
| E-mail(s) |
rfry@unc.edu
|
| Organization name |
UNC-Chapel Hill
|
| Department |
Environmental Sciences and Engineering
|
| Street address |
1213 MHRC
|
| City |
Chapel Hill |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platform ID |
GPL6955 |
| Series (1) |
| GSE22365 |
miRNA expression data from A549 exposed to formaldehyde |
|
