|
| Status |
Public on Aug 08, 2010 |
| Title |
U87 IRE1dn cells replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Human glioblastoma cell (U87) clone: U87 IRE1.NCK DN (U87dn)
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: U87 glioblastoma cell line
genotype/variation: expression of dominant negative transgene of IRE1alpha
|
| Biomaterial provider |
Dr Michel MOENNER
|
| Treatment protocol |
U87 IRE1 dominant negative cells and U87 control cells were grown for 16 hours in culture dishes prior mRNA isolation
|
| Growth protocol |
Cells were incubated for 16h in DMEM supplemented with 10% FBS and glutamine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using RNeasy mini kits (Qiagen) according to the manufacturer's instructions and quantified using a NanoDrop spectrophotometer
|
| Label |
Biotin
|
| Label protocol |
Complementary RNA (cRNA) was prepared according to the manufacturer’s (Affymetrix) 'standard protocol'
|
| |
|
| Hybridization protocol |
Hybridization and washing was carried out on a GeneChip (Affymetrix) station, washing according to the manufacturer’s protocol.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
U87dn cells expressing a dominant negative transgene of IRE1alpha were compared to U87ctrl cells transfected with the corresponding empty plamid to identify genes associated to tumor invasion and angiogenesis.
|
| Data processing |
Data were analyzed with the GCOS 1.2 software (Affymetrix), using the default analysis settings and global scaling as normalization method, with a trimmed mean target intensity value (TGT) of each array arbitrarily set to 100.
|
| |
|
| Submission date |
Jun 16, 2010 |
| Last update date |
Aug 03, 2010 |
| Contact name |
Michel Moenner |
| E-mail(s) |
m.moenner@angio.u-bordeaux1.fr
|
| Phone |
33 540008925
|
| Fax |
33 540008705
|
| URL |
http://www.u-bordeaux1.fr/U920/Site/INSERM_U920.html
|
| Organization name |
Inserm U920
|
| Department |
Université Bordeaux 1
|
| Lab |
Molecular Mechanisms of Angiogensis
|
| Street address |
Avenue des Facultés
|
| City |
Talence |
| ZIP/Postal code |
33405 |
| Country |
France |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE22385 |
Gene profiling: U87 IRE1 dominant negative cells vs. U87ctrl cells in culture |
|
