|
| Status |
Public on Sep 29, 2021 |
| Title |
Cov2_PG1F_6 |
| Sample type |
SRA |
| |
|
| Source name |
Cov2_PG1F_6
|
| Organism |
Mesocricetus auratus |
| Characteristics |
organ: Fat
disease status: Cov2 PG1F
infection: SARS-Cov-2
|
| Treatment protocol |
Mock infected or SARS-CoV-2 treated hamsters
|
| Growth protocol |
Standard Golden Syrian Hamsters
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Fat tissue was harvested and lysed in TRIZOL and subjected to RNA extraction using Trizol extraction protocol
RNA-seq libraries of polyadenylated RNA were prepared using the TruSeq RNA Library Prep Kit v2 (Illumina) or TruSeq Stranded mRNA Library Prep Kit (Illumina) according to the manufacturer’s instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
Lucia90_indexB7
|
| Data processing |
FastQC (v0.11.5) to check FASTQ quality.
STAR alignment (v 2.6.1)
Salmon quantification (0.11.2)
Genome_build: Mesocricetus_auratus (ensembl MesAur1)
Supplementary_files_format_and_content: Gene count matrix
|
| |
|
| Submission date |
Sep 08, 2021 |
| Last update date |
Sep 29, 2021 |
| Contact name |
Sean Houghton |
| E-mail(s) |
seh4002@med.cornell.edu
|
| Organization name |
Weill Cornell Medicine
|
| Street address |
1300 York Ave
|
| City |
New |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL24490 |
| Series (1) |
| GSE183714 |
Hyperglycemia in Acute COVID-19 is Characterized by Adipose Tissue Dysfunction and Insulin Resistance |
|
| Relations |
| BioSample |
SAMN21361358 |
| SRA |
SRX12112764 |
