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Sample GSM5570315 Query DataSets for GSM5570315
Status Public on Sep 12, 2021
Title Atonal_antenna_rep3
Sample type RNA
 
Source name Atonal_antenna
Organism Drosophila melanogaster
Characteristics genotype: Atonal
tissue: drosophila antenna (pool of 60 antennas)
Treatment protocol N/A
Growth protocol Animals were grown at 25°C in 12 h light:12 h dark conditions on standard Drosophila culture media.
Extracted molecule total RNA
Extraction protocol Olfactory third antennal segments were harvested via snap-freezing adult flies with liquid nitrogen and agitating the animals in a mini-sieve. Collected antennae were homogenized manually with a tissue grinder and total RNA was extracted using a standard TRIzol/chloroform protocol.
Label biotin
Label protocol 100 ng RNA was used to perform target preparation using the MessageAmp™ II-Biotin Enhanced aRNA Amplification Kit (ThermoFisher Scientific, Waltham, USA).
 
Hybridization protocol Affymetrix Drosophila Genome 2.0 arrays were hybridized with 11 microgrammes of fragmented biotinylated cRNA , washed, stained and scanned according to the protocol described in Affymetrix GeneChip® Expression Analysis Manual (Fluidics protocol Flex FS450_0002_450)
Scan protocol Scanning was done on an Affymetrix GeneChip Scanner 7G
Description MS_Ato3_7
Data processing Normalized expression signals were calculated from Affymetrix CEL files using RMA (Irizarry et al., 2003) for each tissues separately.
 
Submission date Sep 09, 2021
Last update date Sep 13, 2021
Contact name Sylvain Pradervand
E-mail(s) Sylvain.Pradervand@unil.ch
Phone +41 21 692 39 08
Organization name UNI Lausanne
Department CIG
Lab DNA Array Facility
Street address Genopode
City Lausanne
ZIP/Postal code 1015
Country Switzerland
 
Platform ID GPL1322
Series (1)
GSE183763 Comparative transcriptomic analysis of the Drosophila melanogaster antennal olfactory subsystems.

Data table header descriptions
ID_REF
VALUE Log2 normalized expression signal

Data table
ID_REF VALUE
1616608_a_at 11.93571976
1622892_s_at 8.475893127
1622893_at 12.76217298
1622894_at 7.166386586
1622895_at 7.539777806
1622896_at 7.532743479
1622897_at 3.863359732
1622898_a_at 8.199599997
1622899_at 5.078237283
1622900_at 2.738327822
1622901_at 3.641619948
1622902_at 14.26694845
1622903_s_at 9.275810946
1622904_at 3.970452231
1622905_at 2.996076536
1622906_at 10.17938533
1622907_at 9.138250953
1622908_a_at 4.457486202
1622909_at 8.944094251
1622910_at 2.998506751

Total number of rows: 18952

Table truncated, full table size 432 Kbytes.




Supplementary file Size Download File type/resource
GSM5570315_Ato3_7.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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