|
| Status |
Public on Sep 12, 2021 |
| Title |
WT_antenna_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
WT_antenna
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: Wild-Type
tissue: drosophila antenna (pool of 60 antennas)
|
| Treatment protocol |
N/A
|
| Growth protocol |
Animals were grown at 25°C in 12 h light:12 h dark conditions on standard Drosophila culture media.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Olfactory third antennal segments were harvested via snap-freezing adult flies with liquid nitrogen and agitating the animals in a mini-sieve. Collected antennae were homogenized manually with a tissue grinder and total RNA was extracted using a standard TRIzol/chloroform protocol.
|
| Label |
biotin
|
| Label protocol |
100 ng RNA was used to perform target preparation using the MessageAmp™ II-Biotin Enhanced aRNA Amplification Kit (ThermoFisher Scientific, Waltham, USA).
|
| |
|
| Hybridization protocol |
Affymetrix Drosophila Genome 2.0 arrays were hybridized with 11 microgrammes of fragmented biotinylated cRNA , washed, stained and scanned according to the protocol described in Affymetrix GeneChip® Expression Analysis Manual (Fluidics protocol Flex FS450_0002_450)
|
| Scan protocol |
Scanning was done on an Affymetrix GeneChip Scanner 7G
|
| Description |
MS_Ore2_5
|
| Data processing |
Normalized expression signals were calculated from Affymetrix CEL files using RMA (Irizarry et al., 2003) for each tissues separately.
|
| |
|
| Submission date |
Sep 09, 2021 |
| Last update date |
Sep 13, 2021 |
| Contact name |
Sylvain Pradervand |
| E-mail(s) |
Sylvain.Pradervand@unil.ch
|
| Phone |
+41 21 692 39 08
|
| Organization name |
UNI Lausanne
|
| Department |
CIG
|
| Lab |
DNA Array Facility
|
| Street address |
Genopode
|
| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE183763 |
Comparative transcriptomic analysis of the Drosophila melanogaster antennal olfactory subsystems. |
|
