|
| Status |
Public on Jun 14, 2014 |
| Title |
phc98039 |
| Sample type |
RNA |
| |
|
| Source name |
liver tissue of subject 20, non-tumor
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: liver
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GCS3000 high resolution scanner (M10).
|
| Description |
Gene expresion data from sample phc98039
|
| Data processing |
The data were analyzed with the software of RMAExpress with custom CDF file, quantile normalization, background adjust, and median polish.
|
| |
|
| Submission date |
Jun 17, 2010 |
| Last update date |
Jun 14, 2014 |
| Contact name |
Henry H Zhang |
| Organization name |
NICHD
|
| Department |
NICHD Bioinformatics and Scientific Programming Core
|
| Street address |
49 Convent Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892-4417 |
| Country |
USA |
| |
|
| Platform ID |
GPL10553 |
| Series (1) |
| GSE22405 |
Gene expression profiling of liver cancers |
|
