|
| Status |
Public on Jun 07, 2011 |
| Title |
GATA6 KO ileum, replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
induced, intestine-specific GATA6 knockout mouse ileum, whole
|
| Organism |
Mus musculus |
| Characteristics |
tissue: whole ileum
treatment: tamoxifen induced intestine-specific GATA6 knockout
|
| Treatment protocol |
6-8 wk old mice were treated for 5 consecutive days with 100 mg/ml tamoxifen then killed 28 days later (10-12 wks final age)
|
| Growth protocol |
mice grown under standard conditions, chow diet and water fed ad libitum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy kit (Qiagen) with DNAse treatment
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol for mouse Gene 1.0 ST array
|
| |
|
| Hybridization protocol |
Standard Affymetrix hybridization protocols for the mouse Gene 1.0 ST array
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
| Description |
transcript profile of mouse ileum 28d after tamoxifin induction of transgene deletion, 10 wks at harvest
|
| Data processing |
Data was analyzed using the Affymetrix Expression Console
|
| |
|
| Submission date |
Jun 17, 2010 |
| Last update date |
Jun 07, 2011 |
| Contact name |
James C Fleet |
| E-mail(s) |
james.fleet@austin.texas.edu
|
| Organization name |
University of Texas at Austin
|
| Department |
Nutritional Sciences
|
| Street address |
1400 Barbara Jordan Blvd
|
| City |
Austin |
| State/province |
TX |
| ZIP/Postal code |
78732 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE22416 |
Expression data from ileum of inducible intestine-specific GATA6 knockout mice |
|
