developmental stage (boyes et al. plant cell 2001): emerging tassel
Treatment protocol
no treatment
Growth protocol
ear internode - Crop field Irrigation was applied during a dry and hot summer to prevent water stress
Extracted molecule
total RNA
Extraction protocol
RIL99_pan_1:113.41ng.
Label
Biotin
Label protocol
labelling Biotin direct, amplification=yes, cRNA 20 ug.
Hybridization protocol
labelled extract quantity: 10ug
Scan protocol
GCOS, Biotin:pmt voltage 570nm,650V,laser power 1
Description
Four major QTL explaining lignin content and cell wall degradability variation have been identified in the F288 x F271 RIL progeny (Roussel et al., 2002, Maydica 47: 9-20). Gene expression has been considered as a way in identification of candidate gene underlying QTL. Five lines chosen for their allelic pattern at four main QTL were considered for expression studies, two lines with all four favorable alleles (RIL99, RIL54), two lines with the unfavorable allele at bin 6.06 QTL position (RIL39 , RIL71), and one line with the unfavorable allele at bin 9.02 QTL position (RIL118). Expression studies will be investigated in ear internode at two stages (emerging tassel and early silking).
Data processing
The data were normalized with the gcrma algorithm (Irizarry et al., 2003), available in the Bioconductor package (Gentleman and Carey, 2002).
