|
| Status |
Public on Aug 22, 2024 |
| Title |
R3 |
| Sample type |
RNA |
| |
|
| Source name |
C57BL/6 wildtype
|
| Organism |
Mus musculus |
| Characteristics |
srtrain: C57BL/6
tissues: subcutaneous tumor (melanoma)
cell type: mixture of: melanoma tumor cells and infiltrating immune cells
age: 10 weeks
genotype: Wildtype
treatment: treated with anti-PD-1 + RG7112
|
| Treatment protocol |
Mice were treated intraperitoneally with anti-PD-1 on day 1, 4 and 8 after melanoma injection and treated orally with RG7112 or vehicle (corn oil + 5 % DMSO) every second day for 6 days (two days after B16.F10 melanoma injection)
|
| Growth protocol |
B16.F10 luc+GFP+ melanoma cells were maintained in DMEM (with 10 % FCS and 1 % PenStrep) with a confluency up to 90 % for one week prior to injection
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the miRNeasy Mini kit (Qiagen). RNA quality was assessed by Agilent 2100 Bioanalyzer (Agilent 141 Technologies). RNA samples with an RNA quality number (RQN) greater than 9 were further processed for Affymetrix microarrays.
|
| Label |
Biotin
|
| Label protocol |
We used the Affymetrix Clariom S kit for cDNA synthesis, amplification, and second-cycle cDNA synthesis, as well as fragmentation and labeling with Biotin, as described by the manufacturer.
|
| |
|
| Hybridization protocol |
Labeled fragments were hybridized to Affymetrix Clariom S arrays for 16h at 45°C with 60 rpm in an Affymetrix Hybridization oven 645.
|
| Scan protocol |
After washing and staining, the arrays were scanned with the Affymetrix GeneChip Scanner 3000 7G. CEL-files were produced from the raw data with Affymetrix GeneChip Command Console Software.
|
| Data processing |
CEL files were analysed using the oligo R package. RMA method, log2 transformed were used to normalized the probe intensities.
|
| |
|
| Submission date |
Sep 21, 2021 |
| Last update date |
Aug 22, 2024 |
| Contact name |
Geoffroy Andrieux |
| Organization name |
University clinics Freiburg
|
| Street address |
Breisacherstr 153
|
| City |
Freiburg |
| ZIP/Postal code |
79110 |
| Country |
Germany |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE184524 |
MDM2 inhibition enhances immune checkpoint inhibitor therapy in melanoma |
|
