|
| Status |
Public on Feb 28, 2011 |
| Title |
Placenta_Early_PE_4 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Placenta Early-onset PE
|
| Organism |
Homo sapiens |
| Characteristics |
gestational age (w+d): 26+2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
5 µg of total RNA were labelled using Corning ChipShot labelling system following manufacturer's instructions. Before hybridization, 50pmol Cy3 labelled cDNA from each experimental sample was pooled with 50pmol of reference Cy-5 labelled cDNA
|
| |
|
| Channel 2 |
| Source name |
Total RNA from pooled human term healthy placenta
|
| Organism |
Homo sapiens |
| Characteristics |
gestational age: term
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
5 µg of total RNA were labelled using Corning ChipShot labelling system following manufacturer's instructions. Before hybridization, 50pmol Cy3 labelled cDNA from each experimental sample was pooled with 50pmol of reference Cy-5 labelled cDNA
|
| |
|
| |
| Hybridization protocol |
Arrays were hybridized using Pronto! Microarray Hybridization kit following manufacturer's instructions
|
| Scan protocol |
Scanned on an Agilent G2565AA scanner. Images were quantified using Gene Pix Pro 4.1 software
|
| Description |
Sample values are normalized log ratio (reference/test) data that are comparable across rows and Samples
|
| Data processing |
Data was analayzed using the Bio Array Software Environment (BASE). Spots with intensities below 100 in both of the two channels were excluded. The intensity of the spot also had to be two times as high as the background to be included in further analysis. Using Lowess and global median centering, data was normalized for labelling and reference bias respectively. Fluorescence ratios were calculated as Cy3/Cy5 and log2 transformed.
|
| |
|
| Submission date |
Jun 23, 2010 |
| Last update date |
Feb 28, 2011 |
| Contact name |
Katja Junus |
| Organization name |
Uppsala University
|
| Department |
Department of Women's and Children's Health
|
| Street address |
University Hospital
|
| City |
Uppsala |
| ZIP/Postal code |
SE-751 85 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL10579 |
| Series (1) |
| GSE22526 |
Gene expression profiling of placenta from women with early- and late-onset preeclampsia reveals differences in angiogenesis |
|
