|
| Status |
Public on Sep 15, 2022 |
| Title |
SVF treated, replicate 4 |
| Sample type |
SRA |
| |
|
| Source name |
coronary arterioles
|
| Organism |
Rattus norvegicus |
| Characteristics |
Sex: female
strain: Fischer-344
age: 24 months
treatment: single intravenous injection of 10^7 adipose-derived stromal vascular fraction cells
|
| Treatment protocol |
No treatment for young and old control replicates. Adipose-derived stromal vascular fraction cells were administered to 23 month old female Fisher 344 rats via single tail vein injection of 10^7 cells. Coronary microvessels were fershly haarvested 4 weeks later.
|
| Growth protocol |
Coronary microvessels were freshly isolated from branches off the left anterior descending artery in the apical region.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA was isolated from ≥ 5 microvessels from pooled within the group using RNAeasy Micro plus kit (Qiagen).
Libraries were prepared using the Universal Plus mRNA-Seq with NuQuant (NuGEN(TECAN) Cat# 0520-24).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
AL-12-OSVF-4_S11
|
| Data processing |
Raw sequences were checked for quality values using fastQC v0.10.1. Quality trimming was not deemed necessary.
The sequences were directly aligned to the Rattus norvegicus reference genome assembly (rn6) using Star version 2.6.
Read counts for gene regions were obtained with HTSeq v.0.10.0 using Ensembl annotations
Genome_build: Rn6
Supplementary_files_format_and_content: htseq-count derived matrix table with raw gene counts for every gene and every sample.
|
| |
|
| Submission date |
Sep 23, 2021 |
| Last update date |
Sep 15, 2022 |
| Contact name |
Eric Christian Rouchka |
| E-mail(s) |
eric.rouchka@louisville.edu
|
| Organization name |
University of Louisville
|
| Department |
Biochemistry and Molecular Genetics
|
| Lab |
KY INBRE Bioinformatics Core
|
| Street address |
522 East Gray Street
|
| City |
Louisville |
| State/province |
Kentucky |
| ZIP/Postal code |
40292 |
| Country |
USA |
| |
|
| Platform ID |
GPL20084 |
| Series (1) |
| GSE184674 |
Gene expression profiling of age-related changes in coronary arterioles following intravenous stromal vascular fraction using RNA-seq |
|
| Relations |
| BioSample |
SAMN21572591 |
| SRA |
SRX12312139 |
