|
| Status |
Public on Dec 31, 2010 |
| Title |
OB-control, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
liver of ob/ob mice
|
| Organism |
Mus musculus |
| Characteristics |
strain: B6.V-Lepob/J
genotype: ob/ob
tissue: liver
agent: control
|
| Treatment protocol |
The ob/ob mice were given TUDCA (Tokyo Tanabe, Tokyo, Japan) at a dose of 500 mg/kg twice a day (8:00 AM and 8:00 PM) by gastric gavage for 3 weeks (OB-TUDCA group, n = 3). We administered tap water to control ob/ob mice (OB-control group, n = 3) and normal control C57BL/6J mice (N-control group, n = 3) twice a day by gastric gavage for 3 weeks.
|
| Growth protocol |
Mice ob/ob mice and control littermate (C57BL/6J) were fed a normal chow diet (Purina LabDiet, St Louis, MO, USA) ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The mice were killed after an 8 hour fasting. The livers were immediately retrieved and kept freshly frozen.
|
| Label |
biotin
|
| Label protocol |
The extraction of total RNA was performed according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (GeneChip® Whole Transcript (WT) Sense Target Labeling Assay Manual, version 4, Affymetrix).
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 7G.
|
| Description |
Gene expression data from liver of ob/ob control
|
| Data processing |
Microarray data was normalized with Expression Console software (Affymetrix, Santa Clara), using RMA algorithms.
|
| |
|
| Submission date |
Jun 29, 2010 |
| Last update date |
Dec 31, 2010 |
| Contact name |
Jae-Seong Yang |
| E-mail(s) |
destine@postech.ac.kr
|
| Organization name |
Pohang University of Science and Technology
|
| Department |
School of Interdisciplinary Bioscience and Bioengineering
|
| Lab |
Structural Bioinformatics Laboratory
|
| Street address |
San 31 Hyoja-dong
|
| City |
Pohang |
| ZIP/Postal code |
790-784 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE22608 |
Changes in Hepatic Gene Expression upon Oral Administration of Taurine-Conjugated Ursodeoxycholic Acid in ob/ob Mice |
|
