|
| Status |
Public on Dec 31, 2022 |
| Title |
S7 |
| Sample type |
SRA |
| |
|
| Source name |
SVZ dissected tissue
|
| Organism |
Oryctolagus cuniculus |
| Characteristics |
group: Premature rabbit pups treated with glycerol to induce intraventricular hemorrhage
disease state: Intraventricular Hemorrhage (GMH-IVH)
age: Postnatal day 3
tissue: SVZ disected tissue
|
| Treatment protocol |
Premature rabbit pups were injected intraperitoneal glycerol (50%) to induce intraventricular hemorrhage and compared with healthy control no hemorrhage
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Qiagen RNeasy micro kit was used for RNA extraction from dissected tissue samples collected using microscope from the SVZ region.
Illumina TruSeq Stranded Total RNA Library Prep Kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 550 |
| |
|
| Data processing |
Kallisto for pseudo-alignment to transcriptome of Ensembl OryCun2.0
DESeq2 for differential gene expression
Genome_build: Ensembl OryCun2.0
Supplementary_files_format_and_content: A .csv file including TPM of each gene for all 8 samples
|
| |
|
| Submission date |
Oct 06, 2021 |
| Last update date |
Dec 31, 2022 |
| Contact name |
Weihua Huang |
| E-mail(s) |
huangwe21@ecu.edu
|
| Phone |
2527443748
|
| Organization name |
East Carolina University
|
| Department |
Pathology
|
| Street address |
600 Moye Blvd, Brody 7S-24
|
| City |
Greenville |
| State/province |
North Carolina |
| ZIP/Postal code |
27834 |
| Country |
USA |
| |
|
| Platform ID |
GPL29005 |
| Series (1) |
| GSE185438 |
Germinalmatrix-Intraventricular Hemorrhage induced Early Gene Expression Profile in Subventricular zone (SVZ) of developing rabbit forebrain |
|
| Relations |
| BioSample |
SAMN22091279 |
| SRA |
SRX12501553 |
