|
| Status |
Public on Aug 05, 2022 |
| Title |
LX2 siCTRL #3 |
| Sample type |
RNA |
| |
|
| Source name |
LX2 cells transfected with siCTRL
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: siCTRL
|
| Treatment protocol |
Cells were transfected using JetPrime (Polyplus transfection) with 20 nM siRNA targeting BNC2 (siBNC2, Thermo Fisher Scientific, Cat# 4392420 s29452) or a non-targeting control siRNA (siCTRL, Thermo Fisher Scientific Cat# 4390844)
|
| Growth protocol |
Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 5% fetal bovine serum (FCS, Deutscher).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Trizol and reverse transcribed using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems, USA). Total RNA was checked for quantity and quality using the Agilent 2100 Bioanalyzer (Agilent Biotechnologies).
|
| Label |
biotin
|
| Label protocol |
RNA were processed using Affymetrix GeneChip® WT PLUS Reagent Kit.
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip Hybridization Oven 640 for 16h at 45°C and 60 rpm on HAT 2.0 Chip. GeneChips were washed and stained in the Affymetrix Fluidics.
|
| Scan protocol |
Affymetrix GeneChip Scanner 7G and Affymetrix GeneChip Command Console software (AGCC).
|
| Description |
Transcriptomic LX2 siCTRL
|
| Data processing |
Normalization was performed using the apt-probeset-summarize tool of Affymetrix Power Tools (https://www.affymetrix.com/support/developer/powertools/changelog/index.html) using “gc correction+scale intensity+rma”. Normalization level was set to “Core genes”. The data obtained are log2-transformed.
|
| |
|
| Submission date |
Oct 07, 2021 |
| Last update date |
Aug 05, 2022 |
| Contact name |
Jérôme Eeckhoute |
| E-mail(s) |
jerome.eeckhoute@inserm.fr
|
| Organization name |
CNRS
|
| Lab |
INSERM UMR 1011, Université Lille-Nord de France
|
| Street address |
Bâtiment J&K, Faculté de Médecine de Lille-Pôle Recherche, Boulevard du Professeur Leclerc
|
| City |
Lille |
| ZIP/Postal code |
59045 |
| Country |
France |
| |
|
| Platform ID |
GPL17586 |
| Series (2) |
| GSE185526 |
Transcriptomic analysis of BNC2 regulated genes in LX2 cells |
| GSE185529 |
BNC2 regulated genes in myofibroblasts and liver fibrosis |
|
