|
| Status |
Public on Aug 24, 2022 |
| Title |
Liver_CC_Female_Mice_ID_120 |
| Sample type |
RNA |
| |
|
| Source name |
Liver from CC female mice
|
| Organism |
Mus musculus |
| Characteristics |
gender: Female
mouse type: CC027/GeniUnc
experimental diet: HP
tissue: Liver
|
| Treatment protocol |
Mice were fed either a high protein (HP) or high fat high sucrose (HS) experimental diet for 8 weeks
|
| Growth protocol |
Collaborative Cross (CC) mice between 6-9 weeks of age were obtained from were obtained from University of North Carolina’s CC Mouse Core and maintained on a synthetic chow diet (AIN-76A) for 2 weeks prior to administration of experimental diets
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from about 25 mg of liver tissue using Norgen Total RNA Purification Kit, Norgen
|
| Label |
biotin
|
| Label protocol |
Done according to Affymetrix MoGene 2.1 ST 96-Array Plate Protocol
|
| |
|
| Hybridization protocol |
Done according to Affymetrix MoGene 2.1 ST 96-Array Plate Protocol
|
| Scan protocol |
Done according to Affymetrix MoGene 2.1 ST 96-Array Plate Protocol using the Affymetrix GeneTitan instrument
|
| Description |
Gene expression in liver tissue obtained from female CC mouse
|
| Data processing |
Probes containing known SNPs from the founder strains were masked. We used the robust multiarray average method (RMA) implemented in the Affymetrix gene expression console with default settings (median polish and sketch-quantile normalization) to estimate the normalized expression levels of transcripts.
|
| |
|
| Submission date |
Oct 08, 2021 |
| Last update date |
Aug 24, 2022 |
| Contact name |
Brian J Bennett |
| Organization name |
USDA ARS-WHNRC
|
| Street address |
430 West Health Sciences Drive
|
| City |
Davis |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE185547 |
Genetic background and diet induce hepatic gene expression of different regulatory pathways associated with obesity in the Collaborative Cross |
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