|
| Status |
Public on Aug 02, 2010 |
| Title |
3_WT_poly(I:C) |
| Sample type |
RNA |
| |
|
| Source name |
fetal-liver macrophages
|
| Organism |
Mus musculus |
| Characteristics |
genotype: wild type
treatment: poly(I:C) 8 hours
experiment: 3
cell type: fetal-liver macrophages
|
| Treatment protocol |
on d7 1x10^6 fetal-liver macrophages were given fresh medium (=untreated) or medium containing poly(I:C) (50 µg/ml) for 8 hours.
|
| Growth protocol |
cell suspensions from single fetal livers (d14) were cultured for 5 days in the presence of L929 conditioned medium (as source for M-CSF1), on d6 1x10^6 cells per well were plated in 6-well clusters.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from 1x10^6 cells lysed in 1 ml TRiZOL reagent.
|
| Label |
digoxigenin
|
| Label protocol |
20 µg of total RNA was processed according to the manufacturer's instructions to produce digoxigenin-labelled cDNA
|
| |
|
| Hybridization protocol |
according to the manufacturer's instructions
|
| Scan protocol |
according to the manufacturer's instructions
|
| Description |
ABI 1700 mouse whole genome array
|
| Data processing |
using GeneSpring software; Data transformation and normalisation: expression values less than 10 were set to 10. Data were normalised to the 50th percentile (intra-array normalisation) and each gene was normalised to the median expression (inter-array normalisation). Data were pre-filtered based on the signal to noise ratio (cut-off level: signal above noise > two-fold in all samples of the respective biological replicate group) and non-changing genes (normalised expression levels from 0,667 to 1,334 in at least 4 of 4 conditions) were substracted.
|
| |
|
| Submission date |
Jul 02, 2010 |
| Last update date |
Jul 31, 2010 |
| Contact name |
Elisabeth Hofmann |
| E-mail(s) |
elisabeth.hofmann@vetmeduni.ac.at
|
| Organization name |
University of Veterinary Medicine Vienna
|
| Department |
Institute of Animal Breeding and Genetics
|
| Street address |
Veterinaerplatz 1
|
| City |
Vienna |
| ZIP/Postal code |
A-1210 |
| Country |
Austria |
| |
|
| Platform ID |
GPL2995 |
| Series (1) |
| GSE22691 |
Comparison of the expression profiles of wild type (WT) and Oct-6-deficient (Oct-6-/-) macrophages in response to poly(I:C) treatment |
|
