|
| Status |
Public on Nov 01, 2021 |
| Title |
Sperm DNA from 10017 |
| Sample type |
genomic |
| |
|
| Source name |
Semen Sample 10017
|
| Organism |
Homo sapiens |
| Characteristics |
treatment category: Folic Acid
age: 27
tissue: Somatic cell lysed sperm sample
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA was extracted and purified from sperm samples following somatic cell lysis using Qiagen DNeasy Kit with sperm specific modifications
|
| Label |
biotin
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina's EPIC array using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Data processing |
R (both Base R and the minfi software package in R)
Average Beta
|
| |
|
| Submission date |
Oct 14, 2021 |
| Last update date |
Nov 02, 2021 |
| Contact name |
Tim Jenkins |
| E-mail(s) |
tim_jenkins@byu.edu
|
| Phone |
801 422 6797
|
| Organization name |
Brigham Young University
|
| Street address |
4005 Life Sciences Building
|
| City |
Provo |
| State/province |
UT |
| ZIP/Postal code |
84602 |
| Country |
USA |
| |
|
| Platform ID |
GPL29753 |
| Series (1) |
| GSE185920 |
Epigenetic analysis of sperm DNA methylation from a randomized placebo controled study of zinc and folic acid suplementation |
|
