|
Status |
Public on Nov 01, 2021 |
Title |
Sperm DNA from 10633 |
Sample type |
genomic |
|
|
Source name |
Semen Sample 10633
|
Organism |
Homo sapiens |
Characteristics |
treatment category: Folic Acid age: 35 tissue: Somatic cell lysed sperm sample
|
Extracted molecule |
genomic DNA |
Extraction protocol |
genomic DNA was extracted and purified from sperm samples following somatic cell lysis using Qiagen DNeasy Kit with sperm specific modifications
|
Label |
biotin
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina's EPIC array using standard Illumina protocol
|
Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
Data processing |
R (both Base R and the minfi software package in R) Average Beta
|
|
|
Submission date |
Oct 14, 2021 |
Last update date |
Nov 02, 2021 |
Contact name |
Tim Jenkins |
E-mail(s) |
tim_jenkins@byu.edu
|
Phone |
801 422 6797
|
Organization name |
Brigham Young University
|
Street address |
4005 Life Sciences Building
|
City |
Provo |
State/province |
UT |
ZIP/Postal code |
84602 |
Country |
USA |
|
|
Platform ID |
GPL29753 |
Series (1) |
GSE185920 |
Epigenetic analysis of sperm DNA methylation from a randomized placebo controled study of zinc and folic acid suplementation |
|