|
| Status |
Public on Oct 22, 2021 |
| Title |
Insulin sensitive (IS) 3 |
| Sample type |
RNA |
| |
|
| Source name |
MCF7 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF7
cell type: breast cancer
treatment: Co-cultured with insulin sensitive adipocytes
|
| Treatment protocol |
Cells were co cultured either innsulin sensitye or insulin resistant adipocytes for 5 days. Then RNA of the cells were isolated and cDNA synthesized
|
| Growth protocol |
MCF7 cells were cultured in DMEM including 10% FBS and 1% antibiotics for an over night
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolated using Qiagen RNA purification protocol and reagents
|
| Label |
SYBR Green
|
| Label protocol |
N/A
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
Test
|
| Data processing |
Ct values were generated by ABI 7500 Fast System Software
|
| |
|
| Submission date |
Oct 21, 2021 |
| Last update date |
Oct 22, 2021 |
| Contact name |
Naser Jafari |
| E-mail(s) |
njafari@bu.edu
|
| Phone |
8592309730
|
| Organization name |
Boston University
|
| Department |
Hem Onc
|
| Lab |
Denis lab
|
| Street address |
72 East Concord
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02118 |
| Country |
USA |
| |
|
| Platform ID |
GPL24335 |
| Series (2) |
| GSE186364 |
Real-time quantitative PCR analysis of human EMT genes in MCF-7 cells [Data S1] |
| GSE186432 |
Adipocyte-derived extracellular vesicles promote breast cancer progression in type 2 diabetes |
|
